Aguirre, the Wrath of God, does not feature any identifiable LGBTQ+ characters or themes. The narrative is entirely focused on the historical expedition of conquistadors and their struggle against nature and each other, with no elements pertaining to queer identity or experience.

Aguirre, the Wrath of God is a historical drama centered on a conquistador's descent into madness. The film does not feature any transsexual characters or themes, nor does it touch upon gender identity or transition in its narrative or character portrayals.

The film features two significant female characters, Inés de Atienza and Flores de Aguirre. Neither character participates in any physical combat throughout the film. They are portrayed as non-combatants within the expedition, and there are no scenes depicting them engaging in or winning close-quarters fights against male opponents.

The film is an original screenplay by Werner Herzog, inspired by historical events. All major historical figures and original characters maintain their established or depicted gender, with no instances of a character canonically or historically established as one gender being portrayed as another.

The film is an original screenplay by Werner Herzog, not an adaptation of existing source material with established character races. Major historical figures like Lope de Aguirre are portrayed by actors of the same race as their historical counterparts. No character was canonically, historically, or widely established as one race and then portrayed as a different race.