The film 'AINBO: Spirit of the Amazon' focuses on an environmental adventure and indigenous culture. It does not feature any explicit or implicit LGBTQ+ characters or themes, resulting in no portrayal within its story.

AINBO: Spirit of the Amazon is an animated adventure film centered on a young girl's quest to save her Amazonian village. The narrative focuses on environmental themes and indigenous culture, and does not include any discernible transsexual characters or themes.

The film features Ainbo, a young female protagonist who confronts male antagonists such as loggers and the dark spirit Yacuruna. Her victories are achieved through ranged weapons (bow and arrow), environmental traps, and spiritual powers, rather than direct physical combat or martial arts against male opponents.

AINBO: Spirit of the Amazon is an original animated film featuring characters created specifically for this production. There are no pre-existing canonical characters from source material, history, or prior installments whose gender was altered.

Ainbo: Spirit of the Amazon is an original animated film with characters created specifically for this production. There is no prior source material, historical figures, or previous installments from which characters' races could have been canonically established and then changed.