The action-thriller 'Ambulance' primarily focuses on a bank heist, a high-speed chase, and the relationship between two brothers. The narrative does not include any discernible LGBTQ+ characters or themes, resulting in no portrayal to evaluate.

The film "Ambulance" is an action thriller primarily focused on a bank heist and subsequent high-speed chase. It does not feature any identifiable transsexual characters or themes within its narrative, resulting in a determination of N/A for its portrayal.

The film primarily features male characters in combat roles. The main female character, Cam Thompson, is an EMT and her role is focused on providing medical care, not engaging in physical combat. No other female characters are depicted in close-quarters physical combat defeating male opponents.

Ambulance (2022) is an original film with characters created specifically for this production. There is no prior source material, historical record, or previous installment from which character genders could have been established and subsequently swapped.