Aquaman and the Lost Kingdom does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on Arthur Curry's family, his relationship with his brother Orm, and the conflict with Black Manta, without incorporating elements of queer identity or experience.

Based on available information, Aquaman and the Lost Kingdom does not appear to feature any identifiable transsexual characters or themes. Consequently, there is no portrayal to evaluate, resulting in a net impact of N/A. The film's narrative focuses on its established characters and plot without incorporating these specific elements.

The film features Mera as a prominent female character who participates in combat. However, her victories against male opponents are primarily achieved through her hydrokinetic superpowers, rather than direct physical combat, hand-to-hand fighting, or melee weapon use.

All major characters in Aquaman and the Lost Kingdom retain their established genders from the source material and previous film installments. No canonical characters were portrayed as a different gender.