The film 'Assassin' (2025) does not feature any identifiable LGBTQ+ characters or themes within its storyline or character development. Available information indicates a focus on crime and action, with no explicit portrayal or thematic exploration of the LGBTQ+ community.

The film 'Assassin, 2025' is an action historical drama set in 1932 Shanghai, centered on political and military conflict. Based on all available information, there are no identifiable transsexual characters or themes present in the narrative or character portrayals. The movie's focus is exclusively on resistance, betrayal, violence, and sacrifice in the fight for freedom.

Information regarding specific combat scenes for the movie 'Assassin' (2025) directed by Jiuqin Zhou is not available in the provided data. Therefore, it is not possible to determine if it contains scenes where female characters defeat male opponents in physical combat.

Available information indicates no characters in the 2025 film *Assassin* have an on-screen gender that differs from their established gender in source material or historical records.

The film's casting aligns with its 1932 Shanghai setting, featuring East Asian actors for Chinese and Japanese characters, consistent with their established ethnicity and historical context. No characters established as one race are portrayed by actors of a different race.