Alien vs. Predator is a sci-fi action-horror film centered on a conflict between humans, Aliens, and Predators. The narrative does not feature any identifiable LGBTQ+ characters, relationships, or themes, resulting in no portrayal to evaluate.

AVP: Alien vs. Predator does not feature any identifiable transsexual characters or themes. The film's narrative and character arcs are entirely focused on its science fiction action premise, resulting in no portrayal of transsexual identity.

The film features Lex Woods as the primary female character in action. While she engages in combat against Aliens and the Alien Queen, and allies with a Predator, she does not defeat any male opponents in close-quarters physical combat using skill, strength, or melee weapons.

The film introduces new human and Predator characters, none of whom were previously established with a different gender in source material or prior installments. No existing legacy characters undergo a gender change.

All primary human characters in "Alien vs. Predator" (2004) are original to this film. There are no instances of characters previously established in source material or prior installments as one race being portrayed as a different race.