The film features Jerrod, an openly gay character, whose coming-out journey to his father, Calvin, is a central plot point. While Calvin initially struggles, the narrative ultimately portrays his growth towards acceptance and unconditional love. The overall portrayal is affirming, depicting Jerrod with dignity and highlighting familial and community support.

Barbershop: The Next Cut features Raja, a transgender woman, as a new barber. Her identity is acknowledged and she is integrated into the barbershop community without significant conflict or prejudice. The film's focus remains on broader community issues, making her portrayal present but largely incidental, neither strongly affirming nor denigrating her trans identity.

Christianity is subtly present as a foundational element of the community's values and moral compass, often referenced in dialogue and contributing to the characters' desire for peace and unity. It is consistently portrayed as a positive, guiding influence.

The film features Rashad, a Muslim character, whose faith is depicted as integral to his positive moral character, commitment to family, and efforts to foster peace in the community. His portrayal is consistently respectful and affirming, showcasing Islam as a source of strength and virtue.