The film does not feature any identifiable LGBTQ+ characters or themes. The cross-dressing is solely a plot device for disguise and comedic effect, with no exploration of gender identity or sexual orientation within the narrative.

Big Mommas: Like Father, Like Son features cisgender male characters who cross-dress as women for comedic disguise within an undercover plot. The film does not include any identifiable transsexual characters or engage with themes related to transsexual identity, focusing solely on the situational humor of the disguise.

The film is a comedy primarily featuring male characters in female disguises. While these characters engage in comedic action sequences, their victories over male opponents are typically achieved through slapstick, accidental means, or the use of props, rather than direct physical combat demonstrating skill, strength, or martial arts.

The film features male protagonists disguising themselves as women as part of the plot. This falls under the exclusion for performances involving gender disguise within the story, as their canonical gender remains unchanged.

The film is a sequel in an existing franchise where the main characters, Malcolm Turner/Big Momma and Trent Pierce, maintain their established racial portrayals from previous installments. No characters originally established as a different race are present.