The film features a subtle, implied romantic connection between Dora Milaje warriors Ayo and Aneka, highlighted by a brief hand-holding scene. This portrayal is incidental, neither explicitly confirming nor extensively exploring their queer identity, but also avoiding any negative framing or stereotypes.

The film features multiple female characters, including Shuri (as Black Panther), Okoye, and Nakia, who are depicted engaging in and winning direct physical combat against male opponents, ranging from individual soldiers to powerful adversaries.

Namor, a character typically depicted in Marvel Comics with ambiguous or white-coded features as an Atlantean/human hybrid, is portrayed in the film by a Mexican actor and reimagined with an explicit Indigenous Mesoamerican cultural and racial identity, constituting a race swap.