Calamity explores themes of gender non-conformity through its protagonist, Martha Jane Cannary, who adopts masculine attire and behaviors to defy societal expectations. However, the narrative does not depict or explicitly address transsexual characters or themes, focusing instead on challenging gender roles within a historical context.

The film focuses on Martha Jane Cannary's journey and her development of skills in riding and wagon driving. While she demonstrates bravery and challenges male authority, there are no scenes depicting her physically defeating one or more male opponents in close-quarters combat.

The film depicts the historical figure Martha Jane Cannary (Calamity Jane) as female, consistent with her documented gender. No other major characters are identified as having undergone a gender swap from established source material or history.

The film 'Calamity' is an animated depiction of the historical figure Martha Jane Cannary (Calamity Jane). The character is portrayed as white, consistent with her historical race, thus not constituting a race swap.