The film does not feature any identifiable LGBTQ+ characters or themes. Its narrative focuses on adventure, mysticism, and the quest for treasure, without incorporating elements related to queer identity or experiences.

The film Corto Maltese: The Secret Court of the Arcane does not feature any identifiable transsexual characters or themes. Its plot centers on adventure and political intrigue in early 20th-century Siberia, following Corto Maltese's quest for a treasure amidst various factions. There are no narrative elements or character arcs that depict or address transsexual identity.

The film features female characters, notably Pandora Groovesnore, who are involved in the narrative's events. However, none of these characters are depicted engaging in or winning direct physical combat against one or more male opponents through skill, strength, or martial arts.

The animated film adapts characters from the established Corto Maltese comic series. All significant characters, including Corto Maltese, Rasputin, and Pandora Groovesnore, maintain their canonical genders as depicted in the original source material.

The animated film faithfully adapts the visual depictions of its characters from the original comic series. Key characters like Corto Maltese, Rasputin, and Pandora Groovesnore are portrayed consistent with their established races in the source material.

Not depicted in the film with any significant narrative weight.