Dora and the Lost City of Gold does not feature any identifiable LGBTQ+ characters or themes. The film's narrative is entirely focused on a family-friendly adventure, making the portrayal of LGBTQ+ elements not applicable.

Dora and the Lost City of Gold is an adventure film centered on Dora's journey through the jungle. The movie does not include any transsexual characters or themes, focusing instead on exploration, friendship, and solving ancient mysteries without engaging with transgender identity or related issues.

The film features Dora and other female characters who are resourceful and brave, but their victories against male antagonists are achieved through outsmarting them, navigating environmental hazards, or triggering traps. There are no instances of female characters defeating male opponents in direct physical combat.

The film adapts the animated series "Dora the Explorer." All established characters, including Dora, Diego, Boots, Swiper, and Dora's parents, maintain their original canonical genders from the source material.

Dora and her family, established as Latina in the animated series, are portrayed by Latina/Latino actors in the film. There is no instance where a character's race deviates from their established canonical depiction.