The film portrays Ed Wood's gender non-conformity with empathy and dignity, showcasing his cross-dressing as a personal comfort embraced by his loved ones. It celebrates individuality and includes a non-negatively depicted gay character, Bunny Breckinridge. The overall impact is affirming, presenting eccentricities and non-traditional identities respectfully.

The film Ed Wood portrays the titular character's cross-dressing as a significant and authentic aspect of his identity. The narrative treats his gender expression with empathy and dignity, framing it as a source of personal comfort and self-acceptance. His partner Dolores eventually accepts his cross-dressing, highlighting the film's respectful stance rather than endorsing ridicule.

The film portrays family life through unconventional relationships and a strong emphasis on chosen family, highlighting Ed Wood's bond with his eccentric collaborators and his supportive marriage that embraces his non-traditional gender expression.