This animated film, a product of 1980s children's entertainment, contains no identifiable LGBTQ+ characters or themes. The narrative focuses exclusively on the Ghostbusters' adventures against Prime Evil, without addressing queer identity in any capacity.

The film, an animated series from the 1980s, does not contain any identifiable transsexual characters or themes. Therefore, an evaluation of its portrayal is not applicable.

The film features Tracy the Gorilla as the primary female character in action sequences. Her combat involves super strength against supernatural entities, which is considered a superpower. No human female characters engage in direct physical combat against male opponents, nor does Tracy's combat meet the criteria of skilled physical combat independent of her superpower.

The film features Jake Kong Jr. and Eddie Spencer Jr., who are the sons of the original male characters from the 1975 live-action series. Other significant characters are new creations for the animated series. No established character from the source material has their gender changed.

This film is a direct-to-video movie based on the 1986 animated series, which itself is based on the 1975 live-action series. All established characters maintain their original racial depictions from the source material, with no changes to their race.