First Blood does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the trauma of a Vietnam veteran and his conflict with local authorities, without incorporating elements related to sexual orientation or gender identity. Therefore, the film has no net impact on the portrayal of LGBTQ+ individuals.

The film "First Blood" does not feature any identifiable transsexual characters or themes. The narrative focuses on a Vietnam veteran's struggle with PTSD and his conflict with local authorities, without any elements related to transgender identity.

The film primarily focuses on the male protagonist, John Rambo, and his conflicts with male law enforcement and military personnel. There are no significant female characters depicted engaging in or winning close-quarters physical combat against male opponents.

First Blood is an adaptation of David Morrell's novel. All primary and significant supporting characters, including John Rambo, Sheriff Will Teasle, and Colonel Sam Trautman, retain their original male gender from the source material in the 1982 film adaptation.

Based on the source novel, the main characters, including John Rambo, Sheriff Teasle, and Colonel Trautman, are portrayed by actors whose race aligns with their established descriptions. No character canonically established as one race was depicted as a different race.