There is insufficient information available to assess the portrayal of LGBTQ+ characters or themes in the film 'Friendship, 2001'. The provided details do not contain any relevant plot points or character descriptions to determine a net impact.

There is insufficient information available about the film 'Friendship, 2001' to assess its portrayal of transsexual characters or themes. The absence of plot details or character descriptions prevents any determination of whether such characters are present or how they might be depicted, thus precluding a specific example.

The movie does not contain any action or adventure elements.

Without information regarding source material, prior adaptations, or historical figures for the film 'Friendship, 2001', it is impossible to determine if any character's gender was canonically established and subsequently changed.

There is insufficient information regarding the film's source material or character origins to determine if any character was canonically, historically, or widely established as one race and then portrayed as a different race.