GANTZ:O is a sci-fi action film primarily focused on survival and combat within its established universe. The narrative does not include any discernible LGBTQ+ characters or themes, therefore, there is no portrayal to evaluate.

GANTZ:O does not appear to include any identifiable transsexual characters or themes. The film's narrative is centered on action and survival within the Gantz game, focusing on its core cast and alien threats. There are no plot points or character arcs that depict or engage with transsexual identity, resulting in no net impact on this specific area.

Female characters Reika Shimohira and Anzu Yamasaki engage in combat against male-coded alien opponents. However, their victories are consistently achieved through the use of the Gantz suit's advanced weaponry (X-Gun, Z-Gun) or its significant physical enhancements, which constitute overwhelming technological superiority.

GANTZ:O is a direct animated adaptation of the Gantz manga's Osaka Arc. All major characters, including Kei Kurono and Masaru Kato, retain their established genders from the original source material, with no instances of gender swapping.

GANTZ:O is a Japanese CGI animated film adapting a Japanese manga. The characters, originally depicted as Japanese in the source material, are portrayed as Japanese in the film, with no changes to their established race.