Based on available information, GATAO: Big Brothers (2025) does not appear to feature any identifiable LGBTQ+ characters or themes. Plot descriptions focus on crime action, and no sources mention queer portrayals, leading to a determination of N/A for LGBTQ+ depiction.

There is currently insufficient information available to assess the portrayal of transsexual characters or themes in 'GATAO: Big Brothers, 2025'. Available plot summaries and production details do not mention any such characters or related story elements, making it impossible to determine a net positive, negative, or neutral impact.

Based on available information, there are no described combat scenes where a female character defeats one or more male opponents in direct physical combat. The search results indicate an absence of such portrayals.

The provided information, including plot summaries and cast lists, does not offer sufficient details on character backstories or comparisons to previous franchise installments to determine if any character's on-screen gender differs from their established gender in source material.

No prior source material or earlier franchise entries establish specific ethnicities or appearances for the major characters. All listed cast members are Taiwanese actors of Han Chinese ethnicity, consistent with the film's setting in Taipei, Taiwan. Therefore, no race swap is identified.