Grave of the Fireflies is a poignant war drama centered on the survival of two siblings. The film does not feature any identifiable LGBTQ+ characters or themes, and therefore, no portrayal of queer identity is present within its narrative.

Grave of the Fireflies is a poignant historical drama depicting the devastating impact of World War II on two Japanese children. The film does not feature any identifiable transsexual characters or themes, therefore, no portrayal or impact can be assessed within this framework.

The movie does not contain any action or adventure elements.

Grave of the Fireflies is an adaptation of a semi-autobiographical short story. The film's main characters, Seita and Setsuko, along with other supporting roles, maintain the same genders as established in the original source material. No characters were portrayed with a different gender than their canonical depiction.

Grave of the Fireflies is an animated film adaptation of a Japanese short story, depicting Japanese characters during World War II. The film's portrayal of character races aligns with the source material and historical context, with no instances of a character canonically established as one race being depicted as a different race.