"Hamburger: The Motion Picture" features LGBTQ+ characters Russell and Connie, whose identities are largely exploited for crude, stereotypical humor. Russell is subjected to homophobic jokes and forced into drag, while Connie is portrayed as an aggressive, predatory lesbian. These depictions reinforce harmful stereotypes without critique, leading to a net negative portrayal.

The film features Russell, a male student who desires to be a woman and frequently cross-dresses. His gender expression and identity are consistently portrayed as a source of crude humor and mockery, serving as a running gag throughout the movie. The narrative uses his character to reinforce stereotypes and generate laughs at his expense, lacking any counterbalancing dignity or empathetic exploration.