The film 'Josie and the Pussycats' does not feature any explicit LGBTQ+ characters or themes within its plot or character arcs. The narrative centers on heterosexual relationships and a satire of pop culture, resulting in no direct portrayal of queer identity.

Josie and the Pussycats does not include any discernible transsexual characters or themes. The story centers on a pop music satire, with no elements related to transgender identity or experiences present in the plot or character arcs.

The movie does not contain any action or adventure elements.

The film adapts the Archie Comics characters, and all major characters, including Josie, Melody, Valerie, Alan, Alexander, and Alexandra, retain their established genders from the source material. No canonical gender changes are present.

The film adapts characters from the Archie Comics series. The main characters Josie, Melody, and Valerie, along with supporting roles, maintain their established racial depictions from the source material.