Jungle Cruise is an adventure film that does not include any identifiable transsexual characters or themes within its narrative. Consequently, there is no portrayal of transgender identity to evaluate regarding its net impact.

Dr. Lily Houghton is a resourceful and active character who participates in many action sequences. However, her victories against male opponents are primarily achieved through her intellect, agility, and the use of environmental factors or specific artifacts, rather than direct physical combat or martial arts.

The film introduces new characters and reinterprets the theme park attraction's Trader Sam. The original attraction did not establish specific races for its characters that were then changed in the film. Trader Sam's portrayal by a Mexican actress maintains an indigenous identity, re-imagining a problematic stereotype rather than changing a previously established race.