Kal Ho Naa Ho includes a comedic subplot where a housekeeper mistakenly believes two male friends are in a romantic relationship. The humor stems from this misunderstanding and the men's embarrassment, effectively using the concept of a same-sex relationship as a punchline. This approach reinforces negative stereotypes rather than affirming LGBTQ+ identities.

While initially used as a plot device for perceived difference, the film portrays Christian characters, particularly Aman and his family, with warmth and positivity, emphasizing love and selflessness.

The film subtly affirms the cultural and ethical values often associated with Hinduism within an Indian context. It portrays family, tradition, and love with respect, presenting them as a positive foundation for human relationships without explicit religious depiction.

Sikh characters are depicted as supportive, warm, and integral to the diverse social fabric of the film, without any negative stereotypes or critiques.