The film 'Kino's Journey: Life Goes On' could not be identified, preventing any assessment of its portrayal of LGBTQ+ characters or themes. Therefore, its net impact on LGBTQ+ representation is N/A.

The film "Kino's Journey: Life Goes On" does not feature any identifiable transsexual characters or themes. Its narrative focuses on Kino's travels and observations of various societies and their unique customs, without engaging with transgender identity or experiences.

The film features Kino, the primary female character, who is highly skilled in combat. However, her victories against male opponents are consistently achieved through the use of firearms. There are no clear instances where she decisively defeats one or more male opponents using only hand-to-hand combat, martial arts, or melee weapons.

The main character, Kino, has an intentionally ambiguous gender in the source material, which is maintained in this film. No other established characters from the broader canon have their gender changed in this adaptation.

The animated film maintains the visual character designs consistent with its Japanese light novel and anime source material. No character established as one race in prior canon is depicted as a different race in this adaptation.