Looney Tunes: Back in Action does not include any discernible LGBTQ+ characters or themes. The film's plot centers on a classic animated adventure with no elements pertaining to queer identity or experiences, resulting in an N/A rating for its portrayal.

Looney Tunes: Back in Action does not feature any identifiable transsexual characters or themes. The film's plot centers on a family-friendly adventure to retrieve a magical diamond, with its narrative and character arcs entirely unrelated to gender identity or transsexual experiences.

The movie does not contain any action or adventure elements.

The film features established Looney Tunes characters who maintain their canonical genders. New human characters are original to the film and do not represent gender-swapped versions of pre-existing characters.

The film primarily features anthropomorphic animated characters, for whom the concept of human race does not apply. All significant live-action human characters are original to this film and do not represent pre-established human characters from prior Looney Tunes canon or history. Therefore, no race swaps occurred.