The film "Lucky Luke" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the titular cowboy's adventures in the Wild West, without incorporating elements related to queer identity or experiences, resulting in no depiction.

The film "Lucky Luke" (2009) does not feature any identifiable transsexual characters or themes. The narrative focuses on the adventures of the titular cowboy in the Wild West, with no elements related to transgender identity or experiences present in the storyline.

The film does not feature any female characters engaging in or winning close-quarters physical combat against one or more male opponents. Female characters are present but do not participate in such action sequences.

The film adapts characters from the Lucky Luke comics, such as Lucky Luke, the Dalton Brothers, and Calamity Jane. All major characters retain their canonical gender from the source material in their on-screen portrayal.

The film "Lucky Luke" (2009) adapts the Belgian comic series. Key characters like Lucky Luke and the Dalton Brothers, who are canonically depicted as white in the source material, are portrayed by white actors in the film. No instances of a character established as one race being portrayed as a different race were identified.