Based on available promotional materials, plot synopses, and mainstream reviews, 'Mark' (2025) does not appear to feature any identifiable LGBTQ+ characters or themes. All sources consistently describe the film as a cop thriller without any mention of LGBTQ+ representation.

Based on available information, the film 'Mark, 2025' does not appear to feature any identifiable transsexual characters or themes. The plot is described as an action thriller focusing on a police officer investigating child kidnappings, with no mention of gender identity elements. Therefore, the portrayal is rated as N/A due to no depiction.

The available information, including plot summaries and reviews, does not contain scene-by-scene details or descriptions of combat sequences. Therefore, it is not possible to determine if any female character defeats male opponents in direct physical combat.

The film "Mark" (2025) is an original story with no pre-existing source material, historical basis, or prior adaptations. Consequently, no characters have an established canonical or historical gender that could be altered on screen.

The film "Mark (2025)" is an original contemporary Indian production. All principal actors are South Asian, portraying characters consistent with the film's South Indian setting. There is no indication of the film being an adaptation or reboot where characters had a pre-established different racial identity.