Mavka: The Forest Song does not feature any identifiable LGBTQ+ characters or themes. The narrative is a fantasy romance centered on a heterosexual couple, focusing on folklore, environmental stewardship, and the conflict between humans and nature.

Mavka: The Forest Song does not feature any explicit portrayal of transsexual characters or themes. The narrative focuses on fantasy, folklore, and environmental topics, with no engagement with transgender identities or issues. Therefore, the film has no net impact on the portrayal of transsexual individuals.

The film features Mavka, a forest spirit, who defends her realm using nature-based magic against human intruders. While she successfully repels male opponents, her victories are achieved through magical abilities like controlling plants and animals, rather than direct physical combat skills or martial arts.

The film's main characters, including Mavka, Lucas, and Kylina, maintain their established genders consistent with Ukrainian folklore and the story's narrative inspirations. No characters are portrayed with a different gender than their source material.

The film's major characters and on-screen population are visually depicted with phenotypical traits consistent with their Ukrainian Slavic origins, aligning with the source material and historical context. While some English voice actors are not Slavic, the animated characters themselves are consistently portrayed as Slavic.