The film's content was not provided, therefore no identifiable LGBTQ+ characters or themes could be evaluated. Based on the lack of information, the portrayal is categorized as N/A.

Based on the provided information, the film "幕末美少年録 會津の決死隊" does not appear to feature any identifiable transsexual characters or themes. Consequently, there is no specific portrayal to evaluate, leading to an N/A rating for its net impact.

The movie does not contain any action or adventure elements.

The 1959 film "Загадка Н.Ф.И." is an adaptation of the 1951 operetta "Девушка из Шанхая." Analysis of the main characters in both the source material and the film reveals no instances where a character's established gender was changed for the screen adaptation.

The film "Загадка Н.Ф.И." (The Mystery of N.F.I.) is a 1959 Soviet adaptation of a children's novel. There is no evidence that any character, canonically or implicitly established as one race in the source material, was portrayed by an actor of a different race in this film.