The film 'Percy Jackson & the Olympians: The Lightning Thief' does not include any explicit LGBTQ+ characters or themes. The narrative centers on the mythological quest and heterosexual relationships, resulting in no discernible LGBTQ+ portrayal for evaluation.

The film 'Percy Jackson & the Olympians: The Lightning Thief' does not feature any identifiable transsexual characters or themes. Its narrative and character arcs are entirely focused on its mythological premise, resulting in no portrayal of transsexual identity.

The film features Annabeth Chase, a demigod, who participates in several combat scenes alongside male characters against various monsters. While she engages in a sword fight with a male opponent, Luke, she is ultimately defeated by him. No female character achieves a clear victory against one or more male opponents in direct physical combat.

The film adaptation maintains the established genders of all major and supporting characters as depicted in Rick Riordan's source novel, "The Lightning Thief." No characters canonically established as one gender are portrayed as a different gender on screen.