Based on current data and available information, Pushpa 2: The Rule does not include identifiable LGBTQ+ characters or themes. The film's narrative primarily focuses on action, political intrigue, and family drama, without notable representation or exploration of LGBTQ+ issues, leading to a net impact of N/A.

Based on available information, there is no confirmed depiction or detailed analysis of transsexual characters or themes in 'Pushpa 2 - The Rule.' While a promotional poster led to fan speculation, this does not confirm the presence or portrayal of trans identities within the film's narrative. Therefore, the net impact is currently N/A.

The provided information highlights Srivalli's assertive and influential role, initiating political upheaval and challenging traditional norms through her presence and decisions. However, it does not describe any scenes where she, or any other female character, engages in or is victorious in close-quarters physical combat against male opponents.

The film is a sequel set in India, with characters consistently portrayed by Indian actors in culturally specific roles. There is no indication of any character, established in previous installments or new, being portrayed by an actor of a different race.