Samuel and the Sign is an animated Christian film centered on a young boy's spiritual journey. The film does not feature any identifiable LGBTQ+ characters or themes, thus there is no portrayal to evaluate within the scope of this framework.

The film "Samuel and the Sign" does not feature any transsexual characters or themes. Its narrative centers on a deaf boy's journey of faith, rendering the rubric's criteria for transsexual portrayal inapplicable.

The movie does not contain any action or adventure elements.

The film is an adaptation of the biblical story of Samuel. All major characters, including Samuel and Eli, are portrayed with the same gender as established in the biblical source material.

The film is an animated adaptation of a biblical story. There is no evidence or historical record to suggest that any canonically or historically established character, such as Samuel, was portrayed on screen as a different race than traditionally depicted.