Scary Stories to Tell in the Dark does not include any identifiable LGBTQ+ characters or themes. The narrative centers on a group of heterosexual teenagers, and queer identities or experiences are not depicted or referenced in any capacity throughout the film.

Scary Stories to Tell in the Dark does not feature any identifiable transsexual characters or themes. The narrative centers on horror elements and the fears of its teenage protagonists, without incorporating any aspects of transsexual identity or experience.

The film's female characters, primarily Stella Nicholls and Ruth Steinburg, are protagonists who are terrorized by supernatural entities. Their roles involve investigation, escape, and survival rather than direct physical combat. There are no scenes where a female character defeats one or more male opponents in close-quarters physical combat.

The film adapts various short stories and monsters from the source material. While some monsters are grotesque, none of the named characters or significant entities established with a specific gender in the original books are portrayed as a different gender in the movie.

The film introduces original main characters for its overarching plot. Characters adapted from the source material (short stories) did not have a canonically established race that was altered in the film.