The film, a collection of animated shorts, includes sketches that depict LGBTQ+ characters and themes primarily for comedic effect. These portrayals often rely on stereotypes, such as in the 'Gay Robot' sketch, reducing identity to a source of mockery rather than offering nuanced or affirming representation. The net impact is negative due to the reliance on problematic stereotypes for humor.

Seth MacFarlane's Cavalcade of Cartoon Comedy includes skits that depict transsexual characters. These portrayals are primarily used for crude, stereotypical humor, often employing derogatory language and situations that mock trans identity. The net impact is negative, as the depictions endorse ridicule and degradation without any affirming or critical counterbalance.

The film frequently employs irreverent humor and satire targeting Christian figures, doctrines, and practices, often portraying them as absurd, hypocritical, or dogmatic for comedic effect without offering significant counterbalancing nuance.

The film includes sketches that satirize aspects of Islam, often relying on stereotypes or controversial themes for comedic impact, which can be perceived as reinforcing negative portrayals.

Jewish characters and practices are occasionally depicted through comedic stereotypes or irreverent jokes, contributing to a portrayal that often leans into mockery rather than nuanced understanding.