No information about the film '{四姊妹}' was provided, making it impossible to evaluate its portrayal of LGBTQ+ characters or themes. Consequently, the assessment defaults to N/A due to the absence of any identifiable depiction.

Based on the provided information, there is no indication of transsexual characters or themes in the film {神秘夫人} ({movie}, {1966}). Therefore, an evaluation of its portrayal is not applicable.

The movie does not contain any action or adventure elements.

The film "Four Sisters" (1966) does not appear to be an adaptation of a prior work with established characters of a different gender, nor does it involve historical figures. There is no evidence of canonical characters undergoing a gender swap.

This 1966 Hong Kong Cantonese opera film features a Chinese cast portraying characters consistent with the cultural and racial context of the production. There is no evidence of any character being established as one race and then portrayed as a different race.