Star Trek II: The Wrath of Khan does not include any identifiable LGBTQ+ characters or themes. The narrative focuses on established heterosexual relationships and deep platonic friendships, with no elements that would allow for an assessment of LGBTQ+ portrayal.

Star Trek II: The Wrath of Khan does not feature any identifiable transsexual characters or themes within its plot or character arcs. The film's narrative is centered on a science fiction conflict and personal drama, with no elements related to transsexual identity or experiences present for evaluation.

The film features female characters such as Lt. Saavik and Dr. Carol Marcus. While Lt. Saavik is a Starfleet officer, her role is primarily tactical and intellectual. No female character is depicted engaging in or winning close-quarters physical combat against male opponents.

Star Trek II: The Wrath of Khan features the original cast reprising their roles, with all established characters maintaining their canonical genders from previous installments. No character's gender was altered from their source material.

All primary and legacy characters in Star Trek II: The Wrath of Khan are portrayed by the same actors who established their roles and races in the original series. No character established as one race in prior canon is depicted as a different race in this film.