Stree 2 includes some engagement with queer themes, notably through a deleted scene exploring gender fluidity. However, the film's net impact is negative due to its reliance on ableist, homophobic, and transphobic humor. This problematic portrayal of characters and themes undermines any potential for positive LGBTQ+ representation, presenting queer identity as a source of mockery.

Stree 2 features trans-coded imagery and themes of gender fluidity, but its portrayal is largely problematic. It uses trans identities for shock value and plot devices, reinforcing stereotypes and otherness. The film fails to provide nuanced or empowering representation, reflecting broader issues in Indian cinema regarding trans depictions.

The film is deeply embedded in Hindu folklore, mythology, and rituals, using them as the central framework for its narrative and cultural setting. While it satirizes patriarchal traditions and superstitions prevalent within this cultural context, it does so by utilizing and engaging with the Hindu milieu, rather than condemning the religion itself.