The Bourne Supremacy is an action-thriller centered on espionage and Jason Bourne's quest for identity. The film does not feature any identifiable LGBTQ+ characters or themes, resulting in no depiction of queer identity or experience within its narrative.

The film 'The Bourne Supremacy' does not feature any identifiable transsexual characters or themes. Its narrative is solely focused on espionage, action, and the protagonist's quest for his past identity within a government conspiracy.

The film features several female characters in significant roles, including intelligence operatives and support staff. However, none of these characters engage in or are depicted as victorious in close-quarters physical combat against male opponents. The action sequences primarily involve male characters.

The Bourne Supremacy is a direct sequel that maintains the established genders of its returning characters from the previous film and source novels. No new characters introduced have a prior canonical gender to swap from.

All major and legacy characters in "The Bourne Supremacy" maintain the same race as established in the preceding film or source material. New characters introduced in this installment do not constitute a race swap.