The Last Samurai does not feature any identifiable LGBTQ+ characters or themes within its narrative. The film focuses on a 19th-century American soldier's experiences with samurai culture in Japan, with no elements related to queer identity present in the storyline.

The Last Samurai does not feature any identifiable transsexual characters or themes. The film's narrative centers on an American soldier's experiences with samurai culture in 19th-century Japan, with no elements pertaining to transgender identity or issues.

The film does not feature any female characters engaging in or winning close-quarters physical combat against one or more male opponents. Female roles are primarily non-combatant throughout the narrative.

The Last Samurai features original characters and historical figures. All historical figures depicted or inspiring characters maintain their documented gender, and original characters do not have a pre-established gender to be swapped from.

The Last Samurai is an original story, not an adaptation of pre-existing material with established character races. All major characters are portrayed by actors whose race aligns with the characters as conceived for this film, or with historical figures they represent.