The Love Guru features LGBTQ+ characters whose identities are primarily used as a source of comedic ridicule. The film relies heavily on harmful stereotypes for characters like Jacques 'Le Coq' Grande and Coach Punch Cherkov, presenting their sexuality and mannerisms as punchlines rather than offering dignified or complex portrayals. The net impact is largely negative due to this problematic depiction.

The film features a character whose past sex reassignment surgery is revealed as a comedic twist. This portrayal uses the character's gender identity as a source of mockery, reinforcing harmful stereotypes and reducing their experience to a punchline, resulting in a negative overall impact.

The film satirizes the commercialization of Eastern spirituality through its portrayal of self-help gurus like Pitka. These characters and their Hindu-inspired practices are consistently depicted as foolish, hypocritical, and superficial, with no counterbalancing positive or nuanced representation of the faith itself.