The People's Joker offers a deeply personal and complex portrayal of LGBTQ+ characters, primarily focusing on trans experiences. It explores themes of self-discovery, chosen family, and resistance against oppression, affirming queer identity and community through its protagonist's journey and satirical critique of mainstream culture.

The People's Joker offers a deeply personal and affirming portrayal of transsexual characters and themes. It centers on a trans woman's messy yet authentic journey of self-discovery, fostering a community of queer outcasts. The film uses subversive parody to critique transphobia and societal oppression, validating trans experiences with humor, heart, and unapologetic authenticity.

The film's main character, Joker, traditionally established as a cisgender male in source material, is portrayed on screen as a trans woman, fulfilling the definition of a gender swap.

Ra’s al Ghul, a character canonically depicted as Middle Eastern or North African in source material, is portrayed by David Liebe Hart, a white actor. This constitutes a clear race swap.