The film contains no transsexual characters or themes. SpongeBob's arc affirms his bubbly personality as valid bravery, rejecting the need to become a 'tough he-man,' yet this addresses gender expression broadly rather than transgender identity.

Female characters including Sandy Cheeks and Barb participate minimally in the story without engaging in direct physical combat against male opponents or achieving victories in such encounters. Action sequences feature male characters in trials and chases resolved through non-combative means.

The movie retains the original SpongeBob SquarePants franchise characters with their established genders, voiced by the longstanding cast including Tom Kenny as SpongeBob and Clancy Brown as Mr. Krabs, showing no gender swaps.

The SpongeBob Movie: Search for SquarePants retains the original voice cast for its legacy characters, who are anthropomorphic sea creatures without canonical human racial identities. New characters introduced in the film are original creations, not recasts of established figures from prior installments.