Toy Story 2 does not feature any identifiable LGBTQ+ characters or themes. The film's narrative centers on the adventures of toys, their friendships, and their existential quest for purpose and belonging, without any explicit or implicit depiction of queer identity or experiences.

Toy Story 2 does not feature any identifiable transsexual characters or themes within its story. Consequently, the film has no direct impact, positive or negative, on the portrayal of transsexual identity.

The film features several female characters, including Jessie, Bo Peep, and Mrs. Potato Head. While Jessie is involved in action sequences and the rescue mission, her interactions do not include direct physical combat where she defeats one or more male opponents. The challenges faced are primarily environmental or group efforts.

All established characters from the first film retain their original genders. New characters introduced in Toy Story 2 are consistent with their on-screen portrayal and do not represent a gender swap from any prior canon or source material.

Toy Story 2 is a direct animated sequel where all established characters maintain their original designs and voice actors, and new characters are introduced without altering any prior racial depictions. No race swaps occurred.