The film "Underworld: Rise of the Lycans" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses exclusively on the origins of the Lycan-Vampire war and the heterosexual romance between Lucian and Sonja, with no elements pertaining to queer identity.

The film "Underworld: Rise of the Lycans" focuses on the origins of the war between Vampires and Lycans, specifically the forbidden love between Lucian and Sonja. There are no identifiable transsexual characters or themes present in the narrative, which is centered on supernatural conflict and romance.

This prequel film features established characters like Lucian, Sonja, and Viktor, all of whom maintain their canonical genders as depicted in previous installments of the Underworld series. No characters established as one gender in prior canon are portrayed as a different gender.

This film is a prequel within an established cinematic universe. All major characters, whether returning or newly introduced, maintain racial consistency with their portrayals in previous installments or the established lore. No character originally depicted or canonically established as one race is portrayed as a different race.