Wayne's World features a single, ambiguous dream sequence where Garth dances with a man, presented as a brief, comedic, and surreal moment. This fleeting depiction is neither affirming nor overtly problematic, remaining incidental to the plot and character development, resulting in a neutral overall impact.

Wayne's World features a brief scene where a character's trans identity is used as a punchline. Garth reacts with shock and disgust when a woman he is dancing with reveals she is a man, framing the revelation as a source of humor and discomfort rather than depicting the character with dignity or respect.