Youth in Revolt features Paul Saunders, an openly gay supporting character whose sexuality is depicted as a normal, non-problematic aspect of his identity. His relationship is shown without judgment, and his character arc focuses on personal struggles and artistic pursuits, making his queer identity present but not central to the narrative.

Youth in Revolt includes Lacey, a minor transsexual character, whose identity is presented incidentally as the girlfriend of a supporting character. The film neither significantly affirms nor denigrates her trans identity, treating it as a factual aspect of her character without making it a central theme or source of conflict.

The film portrays Christian adherents, particularly Jerry and Nick's mother, as naive, somewhat foolish, and superficial in their faith, primarily through the protagonist's cynical perspective and the film's comedic satire. There is no significant counterbalancing positive portrayal, reinforcing a mild negative stereotype.