Pan American Highway (1961)

Based on the information provided, the film 'Chico, the Misunderstood Coyote' does not appear to feature any identifiable LGBTQ+ characters or themes. Therefore, its portrayal of LGBTQ+ elements is considered N/A, indicating no depiction.

Due to the lack of available plot details or character information for the film "{患难真情} ({movie}, {1962})", it is not possible to assess the presence or portrayal of transsexual characters or themes. Therefore, the film's net impact on this aspect is categorized as N/A.

The film's title suggests an animated feature centered on an animal protagonist. Based on this, it is highly improbable that the film contains human female characters engaging in and winning close-quarters physical combat against male opponents using martial arts or melee weapons.

This 1961 animated short introduces the character Chico, a male coyote. There is no prior source material or established canon where Chico or any other significant character was depicted as a different gender.

The film's primary character is an animal, a coyote. The concept of 'race' as defined for human characters does not apply to animals, making a race swap impossible within the given definition.