Afro Samurai (2007)

Afro Samurai does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses exclusively on Afro's quest for revenge, with no explicit or implied queer representation within its storyline or character arcs.

The show features Otsuru, a female character who engages in combat against male opponents. However, her victories and combat effectiveness are primarily achieved through advanced technological enhancements and weaponry, rather than through skill-based hand-to-hand combat or melee weapon proficiency.

The anime adaptation of Afro Samurai maintains the established genders of its core characters from the original manga. No characters canonically established as one gender in the source material are portrayed as a different gender in the show.

The characters in the Afro Samurai animated series, including the protagonist Afro, consistently maintain the racial depictions established in the original manga by Takashi Okazaki. No canonical characters were portrayed as a different race.