Teenage Mutant Ninja Turtles: Out of the Shadows (2016)

The film "Teenage Mutant Ninja Turtles: Out of the Shadows" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses entirely on the titular heroes, their human allies, and their battle against various villains, with no elements related to queer identity or experiences.

The film "Teenage Mutant Ninja Turtles: Out of the Shadows" does not feature any identifiable transsexual characters or themes. The narrative focuses on the titular mutant turtles and their human allies battling villains, without engaging with transgender identity or related issues.

The film features female characters like April O'Neil and Karai who are involved in action sequences. However, neither character is depicted achieving a clear victory in close-quarters physical combat against one or more male opponents through skill, strength, or martial arts. April primarily uses her intelligence and is not a direct combatant, while Karai's combat scenes do not show her decisively defeating male adversaries.

All major characters in Teenage Mutant Ninja Turtles: Out of the Shadows retain their historically established genders from the source material and previous adaptations. No canonical male or female characters were portrayed as a different gender.

Key human characters like April O'Neil and Casey Jones are portrayed by actors matching their historically established races. Shredder and Karai are also portrayed by East Asian actors, consistent with their origins. Baxter Stockman's portrayal aligns with a widely established animated version.