The series '101 Dalmatians: The Series' does not include any explicit or implicit LGBTQ+ characters or themes. Its narrative focuses on the adventures of the Dalmatian puppies and their interactions with other animals and Cruella De Vil, without addressing queer identity in any capacity.

The animated series "101 Dalmatians: The Series" does not feature any identifiable transsexual characters or themes within its narrative. Consequently, there is no portrayal to evaluate regarding transsexual identity or experiences.

The series primarily features comedic and slapstick conflicts where characters, both male and female, often outsmart opponents or cause accidents rather than engaging in direct physical combat. No instances were found where a female character defeats one or more male opponents through skill, strength, or martial arts in close-quarters physical confrontation.

The 1997 animated series maintains the established genders of all core characters from the original 1961 film and 1956 novel, including Cruella De Vil, Pongo, Perdita, Roger, and Anita. No canonical characters undergo a gender change.

The animated series maintains the established racial depictions of its human characters (Roger, Anita, Cruella, Jasper, Horace) from the original 1961 animated film and novel. No characters established as one race in prior canon are portrayed as a different race in this adaptation.